COMPREHENSIVE REVIEW ON COMMONLY USED CULTURE MEDIUM FOR THE DIAGNOSE OF DIFFERENT SPECIES OF BACTERIA AND FUNGUS

Author Name: *Shawana Huma,**Ramsha Zahra Muhammad Muzammal, ***Rizwan, ****Sana Fatima, *****Aiman Nayaband Muhammad Kamran

Volume: 01 &  Issue:

Country: PAKISTAN

DOI NO.: NA, DOI Link: http://doi-ds.org/doilink/08.2020-25662434/

Affiliation:

1. Gomal Centre of Biochemistry and Biotechnology, Gomal University, Dera Ismail Khan, KPK, Pakistan.
   E-Mail: shawanairfan@gmail.com

ABSTRACT

Microbes are the unicellular microscopic organisms. They are found in the form of colonies or as a single cell. Naturally microbes are present everywhere on earth but they can also culture in laboratory. For the growth of microbes commercially available culture media are very expensive and mostly are not available. So researchers are trying to use low cast bio-products for the preparation of culture media. Main components of media are Carbon, Nitrogen, Sulphur, Phosphorous and many other components like minerals etc. Culture media are in different states i.e. liquid, semi-solid, solid that are used in growth and identification of microbes in the lab. Bacteria can grow in solid or liquid media however fungi can be grown on media that are enriched with carbohydrates and nitrogen components with acidic pH of five to six and at temperature 15-37°C. Mainly there are two culturing media for the growth of fungi, one is natural media that is composed of natural components such as seeds, leaves, wheat germ etc, it’s exact composition is unknown but still it is easy to prepare it while other is synthetic media whose composition is known. Both types of media must contain the source of carbohydrates, N and vitamins. Bacteria can grow on different types of media such as Simple media, Complex media, and Synthetic media. In this review we will discuss different media used in the laboratory for growth and diagnosis of Bacteria and fungus. This review will help the researchers in the field of Microbiology.

Key words: culture media,identification,Bacteria,carbohydrates