ISOLATION AND IDENTIFICATION OF FUNGI FROM ORAL OF CANCER PATIENTS AND EVALUATE OF ITS RESISTANCE OF SOME ANTIFUNGAL AGENTS

Author Name: 1. Ghofran Filaih Abd AL-Hussan 2. Ali Yas Khudhair AL-Ameri

Volume/Issue: 02/07

Country: Iraq

DOI NO.: 08.2020-25662434 DOI Link: https://www.doi-ds.org/doilink/01.2022-68718642/UIJIR

Affiliation:

1. Department of Laboratory Investigation, Kufa University, Najaf , IRAQ .
2. Department  of  Medical   Laboratory Technology , Faculty of Medical Technology, The Islamic University, Najaf , IRAQ .

ABSTRACT

This study was conduct to investigate phenotypic identification of fungal species isolated from oral of cancer patients in Al-Najaf governorate and evaluate inhibition activity of some antifungal agents. During the period from January to Februry 2021, 50 isolates were collected from Middle Euphrates Cancer Center in AL_Najaf province, which included swabs from the oral cavity, only 12 isolates gave positive growth. Cultivation of all 12 isolates initially on potato dextrose agar and then diagnosed on chrom agar medium depending on the color of the colonies, finally evaluate some antifungal on growth of diagnosed fungi by using well diffusion method. After isolation and identification the results showed that all isolaled belonged to genus candida these are : C. albicans 6 (50%) , C. Parapsilosis 3( 25%), C. kruzei 2(16.6%) and C. glabrata 1 (8.3%), the ability of C. albicans and non albicans were tested for produce some virulence factors such as germ tube , biofilms and production of enzymes phospholipase when culturing Candida spp. on different media. The results of the culture show that Candida albicans produces all virulence factor that mentioned above .Also the results showed the antifungal activity for (Nystatin and Amphotercin B) , explaining the effects against Candida spp. We can consider the species of yeast are the most prevalent fungal pathogenetic cause of soul invading pathogens in cancer sufferers who are extremely immunocompromised or those who have undergone severe trauma and therapy that necessitates prolonged stays in acute care units. Collect the samples from oral cancer patients, isolation and identification of fungal species on CHROM agar Candida media ,Study some virulence factors of Candida species and Study the effects of some antifungal on growth of Candida species .

Key words: Fungi, Cancer, Patients, Antifungal